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Extracellular Vesicles in Health and Disease

This course is open for applications from May 1 – May 31

EVs have been shown to be released from all three domains of life. In recent years, EVs have been recognized to play an essential role in health and disease by trafficking bioactive molecules between cells. This way, EVs are important in disease progression since both diseased cells and pathogens release EVs. This also suggests that EVs can be used as diagnostic markers for such conditions. The therapeutic potential of EVs ('native form' or modified as drug delivery systems) and their use as vaccines are also currently being explored, all underpinning the interdisciplinary nature of EV research.

In this course, we will cover basic aspects of EVs, which will include their nomenclature, biogenesis, release and uptake, as well as EV cargos. Different isolation methods will be introduced, such as ultracentrifugation, size exclusion chromatography and precipitation techniques, and their pros and cons will be discussed. Different methods to identify, characterize and enumerate EVs will be presented and discussed as well as how to explore their content. As it is not a trivial task to work with EVs, we will discuss critical considerations during the collection and isolation of EVs from various sample types, including non-model organisms. We will then discuss how to explore EV function in vitro and in vivo and, lastly the diagnostic and therapeutic potential of EVs (e.g. as drug delivery systems, vaccines).

During the practical sessions, you will learn how to isolate EVs using size exclusion chromatography (qEV). The size distribution and numbers of EVs isolated will be determined using nanoparticle tracking analysis (NTA) and EV uptake in cells in vitro. EV proteomes and presence of classical EV makers will be assessed using proteomics.

Though the focus of the course will be on mouse and human EVs, we will also cover and discuss EVs from non-model organisms, including outer membrane vesicles (OMVs) and some of their specific challenges and opportunities (e.g. drug delivery system). As many of the methods and considerations for working with EVs are the same irrespectively of their source, this course is relevant for most people interested or already active in the EV field.

Exam info and full course description

Exam info and full course description can be found in the course catalogue.

Please notice that this course is passed by active participation, and you will not receive a specific grade. Only pass/fail will appear on your transcript.

Admission Requirements

Course specific:

The course is offered as a master's-level course and is open to Danish and international students with a Bachelor's degree within health sciences (e.g. medicine, public health, dentistry, sport science).

The course is also offered to national and international PhD students but will still be taught as a master´s-level course. Since EV research is a new and interdisciplinary field that covers several scientific fields we will give a general introduction to the different aspects of EV research and therefore make the course accessible and suitable for both master and PhD students.

It is recommended that the students have basic knowledge of molecular biology and immunology. If you are in doubt about whether you are eligible or how demanding the course is, you are advised to contact the course responsible.


Exchange Students: nomination from your home university

Freemovers: documentation for English Language proficiency

You can read more about the admission here.